Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method Turkey

Improved DOP-PCR (iDOP-PCR): A robust and simple WGA

Classification:Chemical Auxiliary Agent, Chemical Auxiliary Agent
cas no 117-84-0
Other Names:DOP, diocty phthalate, 1,2-phthalate
MF:C6H4(COOC8H17)2
EINECS No.:201-557-4
Purity:99.5%
Type:Plastic Auxiliary Agents
Usage:Plastic Auxiliary Agents, Rubber Auxiliary Agents
MOQ:200kgs
Package:200kgs/battle
Payment:T/T

Here we describe an improved DOP-PCR (iDOP-PCR). We have modified the classic DOP-PCR by using a new thermostable DNA polymerase (SD polymerase) with a

In one study, DOP-PCR failed with low DNA amounts, and an improved DOP-PCR method (called iDOP-PCR), while achieving sensitivity down to 15 pg, showed high proportions of ADOs (46%) and ADIs (4%

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Improved DOP-PCR (iDOP-PCR): a robust and simple WGA

Classification:Chemical Auxiliary Agent, Chemical Auxiliary Agent
cas no 117-84-0
Other Names:DOP, diocty phthalate, 1,2-phthalate
MF:C6H4(COOC8H17)2
EINECS No.:201-557-4
Purity:99.5%, 99.5%
Type:Carbon Black
Usage:Plastic Auxiliary Agents
MOQ:200kgs
Package:200kgs/battle
Place of Origin::China
Advantage:Stable

Improved DOP-PCR (iDOP-PCR): a robust and simple WGA method for efficient amplification of low copy number genomic DNA

iDOP-PCR provided a better quality of the amplified DNA libraries compared to the other WGA methods tested, especially when low amounts of genomic DNA were used as an

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Improved DOP-PCR (iDOP-PCR): A robust and simple WGA

Classification:Chemical Auxiliary Agent
CAS No.:117-84-0
Other Names:DiOctyle Phthalate DOP
MF:C24H38O4
EINECS No.:201-557-4
Purity:99.99, 99%
Type:Adsorbent, Carbon Black
Usage:Coating Auxiliary Agents, Leather Auxiliary Agents, Petroleum Additives, Plastic Auxiliary Agents, Rubber Auxiliary Agents, Surfactants, Textile Auxiliary Agents
MOQ:200kgs
Package:200kgs/battle
Place of Origin::China
Advantage:Stable

Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA. Konstantin A Blagodatskikh. We found that pooling the raw

Whole-genome amplification (WGA) techniques are used for non-specific amplification of low-copy number DNA, and especially for single-cell genome and transcriptome amplification.

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Improved DOP-PCR (iDOP-PCR): A robust and simple WGA

Classification:Chemical Auxiliary Agent
CAS No.:117-84-0
Other Names:DOP, diocty phthalate, 1,2-phthalate
MF:C24H38O4
EINECS No.:201-557-4
Purity:≥99.5%
Type:Plasticizer, Dioctyl Phthalate
Usage:Rubber Auxiliary Agents
MOQ:200kgs
Package:200kgs/battle
Advantage:Stable

Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA. Konstantin A Blagodatskikh, Vladimir M Kramarov, Ekaterina

PCR (DOP-PCR) [7], multiple annealing and looping based amplification cycles (MALBAC) [8] and the PicoPlex technique [9]. The principle of DOP-PCR is to use a single primer con-taining a

Online Inquiry

Improved DOP-PCR (iDOP-PCR): a robust and simple

Classification:Chemical Auxiliary Agent, Chemical Auxiliary Agent
cas no 117-84-0
Other Names:DOP
MF:C6H4(COOC8H17)2
EINECS No.:201-557-4
Purity:99.5%, 99.5%
Type:Adsorbent, plasticizer
Usage:Leather Auxiliary Agents, Paper Chemicals, Petroleum Additives, Plastic Auxiliary Agents, Rubber Auxiliary Agents, Textile Auxiliary Agents, Leather Auxiliary Agent,Plastic Auxiliary Agent,
MOQ::10 Tons
Package:25kg/drum
Place of Origin::China
Advantage:Stable

2 27 Abstract 28 Whole-genome amplification (WGA) techniques are used for non-specific amplification of low-copy 29 number DNA, and especially for single-cell genome and

Mentioning: 2 Whole-genome amplification (WGA) techniques are used for non-specific amplification of low-copy number DNA, and especially for single-cell genome and transcriptome amplification. There are a number of WGA methods that have been developed over the years. One example is degenerate oligonucleotide-primed PCR (DOP-PCR), which is a very simple,

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Is DOP-PCR a good method for WGA?
Although DOP-PCR has been regarded as one of the pioneering methods for WGA, it only provides low genome coverage and a high allele dropout rate when compared to more modern techniques. Here we describe an improved DOP-PCR (iDOP-PCR).
Why is IDOP-PCR better than other WGA methods?
In summary, iDOP-PCR provided a better quality of the amplified DNA libraries compared to the other WGA methods tested, especially when low amounts of genomic DNA were used as an input material.
What is improved DOP-PCR (IDOP-PCR)?
Here we describe an improved DOP-PCR (iDOP-PCR). We have modified the classic DOP-PCR by using a new thermostable DNA polymerase (SD polymerase) with a strong strand-displacement activity and by adjustments in primers design.
What does IDOP PCR look like?
The chromosomes are shown in alternating red and blue colors. At first glance, iDOP-PCR looks like a slightly different variant of DOP-PCR method, but the use of DNA polymerase with a strong strand-displacement activity, instead of Taq polymerase, allows to extremely enhance WGA performance of the method.
Does IDOP-PCR improve the quality of amplified DNA libraries?
The amplified DNA libraries were evaluated by analysis of short tandem repeat genotypes and NGS data. In summary, iDOP-PCR provided a better quality of the amplified DNA libraries compared to the other WGA methods tested, especially when low amounts of genomic DNA were used as an input material.
How does DOP PCR work?
The principle of DOP-PCR is to use a single primer containing a central random sequence. DOP-PCR begins with a few pre-amplification cycles at a low initial annealing temperature, facilitating random primer annealing. Pre-amplification is then followed by PCR amplification of these initial DNA fragments.